microbial strain enterococcus faecalis atcc 33186 Search Results


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ATCC streptococcus faecalis atcc 33186
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ATCC e faecalis
Sources of the 159 enterococcal clinical isolates used in this study
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ATCC e faecalis atcc
Sources of the 159 enterococcal clinical isolates used in this study
E Faecalis Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Sources of the 159 enterococcal clinical isolates used in this study

Journal:

Article Title: Development of a PCR Assay for Rapid Detection of Enterococci

doi:

Figure Lengend Snippet: Sources of the 159 enterococcal clinical isolates used in this study

Article Snippet: These strains represent the following species: E. avium (ATCC 14025), E. casseliflavus (ATCC 25788), E. cecorum (ATCC 43198), E. dispar (ATCC 51266), E. durans (ATCC 19432), E. faecalis (ATCC 19433, ATCC 29212, ATCC 33186, ATCC 49533, and ATCC 51299), E. faecium (ATCC 19434 and ATCC 51559), E. flavescens (ATCC 49996), E. gallinarum (ATCC 49573), E. hirae (ATCC 8043), E. mundtii (ATCC 43186), E. pseudoavium (ATCC 49372), E. raffinosus (ATCC 49427), E. saccharolyticus (ATCC 43076), and E. solitarius (ATCC 49428).

Techniques:

Specificity test performed with the Enterococcus -specific 40-cycle PCR assay and DNA from a variety of gram-positive and gram-negative bacterial species

Journal:

Article Title: Development of a PCR Assay for Rapid Detection of Enterococci

doi:

Figure Lengend Snippet: Specificity test performed with the Enterococcus -specific 40-cycle PCR assay and DNA from a variety of gram-positive and gram-negative bacterial species

Article Snippet: These strains represent the following species: E. avium (ATCC 14025), E. casseliflavus (ATCC 25788), E. cecorum (ATCC 43198), E. dispar (ATCC 51266), E. durans (ATCC 19432), E. faecalis (ATCC 19433, ATCC 29212, ATCC 33186, ATCC 49533, and ATCC 51299), E. faecium (ATCC 19434 and ATCC 51559), E. flavescens (ATCC 49996), E. gallinarum (ATCC 49573), E. hirae (ATCC 8043), E. mundtii (ATCC 43186), E. pseudoavium (ATCC 49372), E. raffinosus (ATCC 49427), E. saccharolyticus (ATCC 43076), and E. solitarius (ATCC 49428).

Techniques:

Example of multiplex PCR amplifications with the Enterococcus-specific PCR assay and the internal control. PCR assays (40 cycles) were performed with 1 μl of genomic DNA (1 ng/μl) from various bacteria and 4,000 copies of linearized pSL1180 as an internal control. The internal control was not amplified when target DNA was present due to competitive inhibition by amplification of Enterococcus DNA. Lanes: 2, E. avium ATCC 14025; 3, E. casseliflavus ATCC 25788; 4, E. cecorum ATCC 43198; 5, E. durans ATCC 19432; 6, E. dispar ATCC 51266; 7, E. faecalis ATCC 19433; 8, E. faecium ATCC 19434; 9, E. flavescens ATCC 49996; 10, E. gallinarum ATCC 49573; 11, E. hirae ATCC 8043; 12, E. mundtii ATCC 43186; 13, E. pseudoavium ATCC 49372; 14, E. raffinosus ATCC 49427; 15, E. saccharolyticus ATCC 43076; 16, E. solitarius ATCC 49428; 17, E. coli ATCC 25922; 1 and 18, controls to which no DNA was added; M, 100-bp molecular size standard ladder.

Journal:

Article Title: Development of a PCR Assay for Rapid Detection of Enterococci

doi:

Figure Lengend Snippet: Example of multiplex PCR amplifications with the Enterococcus-specific PCR assay and the internal control. PCR assays (40 cycles) were performed with 1 μl of genomic DNA (1 ng/μl) from various bacteria and 4,000 copies of linearized pSL1180 as an internal control. The internal control was not amplified when target DNA was present due to competitive inhibition by amplification of Enterococcus DNA. Lanes: 2, E. avium ATCC 14025; 3, E. casseliflavus ATCC 25788; 4, E. cecorum ATCC 43198; 5, E. durans ATCC 19432; 6, E. dispar ATCC 51266; 7, E. faecalis ATCC 19433; 8, E. faecium ATCC 19434; 9, E. flavescens ATCC 49996; 10, E. gallinarum ATCC 49573; 11, E. hirae ATCC 8043; 12, E. mundtii ATCC 43186; 13, E. pseudoavium ATCC 49372; 14, E. raffinosus ATCC 49427; 15, E. saccharolyticus ATCC 43076; 16, E. solitarius ATCC 49428; 17, E. coli ATCC 25922; 1 and 18, controls to which no DNA was added; M, 100-bp molecular size standard ladder.

Article Snippet: These strains represent the following species: E. avium (ATCC 14025), E. casseliflavus (ATCC 25788), E. cecorum (ATCC 43198), E. dispar (ATCC 51266), E. durans (ATCC 19432), E. faecalis (ATCC 19433, ATCC 29212, ATCC 33186, ATCC 49533, and ATCC 51299), E. faecium (ATCC 19434 and ATCC 51559), E. flavescens (ATCC 49996), E. gallinarum (ATCC 49573), E. hirae (ATCC 8043), E. mundtii (ATCC 43186), E. pseudoavium (ATCC 49372), E. raffinosus (ATCC 49427), E. saccharolyticus (ATCC 43076), and E. solitarius (ATCC 49428).

Techniques: Multiplex Assay, Amplification, Inhibition

Detection limits of the Enterococcus -specific PCR assays for 14 enterococcal species

Journal:

Article Title: Development of a PCR Assay for Rapid Detection of Enterococci

doi:

Figure Lengend Snippet: Detection limits of the Enterococcus -specific PCR assays for 14 enterococcal species

Article Snippet: These strains represent the following species: E. avium (ATCC 14025), E. casseliflavus (ATCC 25788), E. cecorum (ATCC 43198), E. dispar (ATCC 51266), E. durans (ATCC 19432), E. faecalis (ATCC 19433, ATCC 29212, ATCC 33186, ATCC 49533, and ATCC 51299), E. faecium (ATCC 19434 and ATCC 51559), E. flavescens (ATCC 49996), E. gallinarum (ATCC 49573), E. hirae (ATCC 8043), E. mundtii (ATCC 43186), E. pseudoavium (ATCC 49372), E. raffinosus (ATCC 49427), E. saccharolyticus (ATCC 43076), and E. solitarius (ATCC 49428).

Techniques: